After setting up Barca team-mate Philippe Coutinho and scoring himself, five-time Ballon d'Or victor Messi landed heavily on his right arm before leaving the field in the first half at Camp Nou.
The Liga giants have announced that their star man will be out for approximately three weeks.
The Chilean's explanation to Valverde wasn't enough to convince him and so the midfielder was left on the bench for the entirety of the match against Sevilla on Saturday night. "We know what he gives us and we know what he does to the opposition".
Marc Andre Ter Stegen started the match on the back of five straight games without a clean sheet.
But Ernesto Valverde could not hide his concern over the prolonged absence of his skipper.
Messi had scored minutes before his injury, doubling Barca's lead against Sevilla after Philippe Coutinho's early opener.
In this period Messi is also set to miss two UEFA Champions League games against Italian club Inter Milan, scheduled to be held on October 24 and November 6.
However, Inter believe it is a great shame that Messi might not feature against them and replied to Barcelona with a wonderful tweet.
Pablo Sarabia finally broke Sevilla through with a goal in the 79th minute with a shot that deflected off Barcelona defender Clement Lenglet. Suarez buried the penalty.
Real Madrid have under-performed under new manager Julen Lopetegui.
The club's previous worst run had been 464 minutes, in 1985.
Atletico Madrid, which drew at Villarreal 1-1, also was two back after nine matches.
"Right now that is the last thing that I'm thinking about", said Lopetegui when asked if he was anxious about losing his job.
"With this initiative, we continue to fight against the cruel disease that is cancer.Collaborating makes this easier".