Mr Zuma has survived several previous votes of no-confidence. "It is a vote against the kleptocracy President Jacob Zuma has actively developed during his tenure as the leader of the ANC and president of the country", Khoza said.
The rand posted its biggest gain in nearly a month, jumping as much as 2 percent before trading 1.4 percent stronger at 13.2636 per dollar by 4:33 p.m.in Johannesburg.
In fixed income, the yield for the benchmark government bond due in 2026 was down 0.5 basis points at 8.57%.
"We would expect a very strong positive reaction in South African asset prices tomorrow if the vote passes, but after that we may be into a period of much more extreme volatility", Attard Montalto said.
He said if Mbete failed to deliver reasons that were consistent with the ConCourt the DA would start processes to challenge her decision.
The motion says investor confidence plummeted after the president axed former finance minister Pravin Gordhan and his deputy Mcebisi Jonas and this plunged South Africa into an economic crisis.
The ANC in Parliament have iterated that they will support the decision the Speaker and were not adverse to either a secret or open ballot. Business Leadership South Africa, (BLSA), has nailed it's colours to the SA mast, advising members to give their staff time off on Tuesday to join the mass protest without losing pay or any benefits.
Both Holomisa and Vavi had criticised the ruling party for continuing to keep Zuma in his position, at the cost of losing the trust and faith of millions of South Africans who were unemployed and those who had decided not to take part in the local government elections in August.
DA chief whip John Steenhuisen said Mbete has left "this announcement to the last possible moment in order to make this about the secret ballot rather than Zuma".
"We can't afford now more scandal in this country", he said.
"Our decision to vote against this motion must therefore not be seen as defending or protecting any individual".
The ANC has ruled Africa's most-industrialized economy since apartheid ended in 1994 and has a 62 percent majority in the National Assembly.
At least 50 of the ANC's 249 MPs need to vote with the opposition to remove President Zuma from office today.
If he is ousted on Tuesday, "all hell would break out within the party ahead of its conference in December", said Roger Southall, a professor at the University of the Witwatersrand in Johannesburg.
A long power struggle could also ensue if the ANC executive tried to ensure victory for its preferred candidate by replacing current MPs with new loyal members.